Expression and DNA binding of budgerigar fledgling disease virus large T antigen

Budgerigar fledgling disease (papovavirus) in pet birds.

References 5. Higuchi R: 1989, Rapid, efficient DNA extraction for PCR from cells or blood. Amplifications 2:1-3. 6. Kibenge FSB, Jackwood DJ, Mercado CC: 1990, Nucleotide sequence analysis of genome segment A of infectious bursal disease virus. J Gen Virol 71:569-577. 7. Kusukawa N, Uemori T, Asada K, Kato I: 1990, Rapid and reliable protocol for direct sequencing of material amplified by the ...

Oligomerization and origin DNA-binding activity of simian virus 40 large T antigen.

Simian virus 40 (SV40) large tumor antigen (T antigen) exists in multiple molecular forms, some of which are separable by zone velocity sedimentation of soluble extracts from infected monkey cells. Three subclasses of this antigen from SV40-infected monkey cells have been separated and characterized: the 5S, 7S, and 14S forms. Newly synthesized T antigen occurs primarily in the 5S form. Chemica...

Structure of the origin-binding domain of simian virus 40 large T antigen bound to DNA.

The large T antigen (T-ag) protein binds to and activates DNA replication from the origin of DNA replication (ori) in simian virus 40 (SV40). Here, we determined the crystal structures of the T-ag origin-binding domain (OBD) in apo form, and bound to either a 17 bp palindrome (sites 1 and 3) or a 23 bp ori DNA palindrome comprising all four GAGGC binding sites for OBD. The T-ag OBDs were shown ...

A Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA

Quantitative analysis of the binding of simian virus 40 large T antigen to DNA.

SV40 large T antigen (T-ag) is a multifunctional protein that successively binds to 5'-GAGGC-3' sequences in the viral origin of replication, melts the origin, unwinds DNA ahead of the replication fork, and interacts with host DNA replication factors to promote replication of the simian virus 40 genome. The transition of T-ag from a sequence-specific binding protein to a nonspecific helicase in...